We compared the two methods of thawing in a water bath and in the Eppendorf SmartBlock™ cryo thaw with various cell lines and presented the results in performance sheets. HEK293, CHO-K1, S16, episomal human iPSC and mono-allelic mEGFP-tagged TOMM20 WTC hiPSC cells were used. All tests showed comparable or better cell viability than the water bath after the thawing process. The deviations of the mean value of the cell viability were mostly lower when using the SmartBlock cryo thaw than when using the water bath. In the case of mono-allelic mEGFP-tagged TOMM20 WTC hiPS cells, pluripotency could also be detected after 4 successful passages after the thawing process in the Eppendorf ThermoMixer® C with Eppendorf SmartBlock cryo thaw.