Programming of the thermocycler
Programming an instrument can prove to be time-consuming as well as error-prone. Different PCR thermal cycler features work together to simplify this process step and, most importantly, to make it safe:
Easy operation and good user guidance are the basis of efficient programming. Building on this foundation, password-protected user administration will prevent one’s own programs from being altered by other users. If multiple cyclers (of the same type) are in use, it is beneficial if a program can be transferred directly from one instrument to another via USB or connectivity. Computer software enables central and secure administration of programs, user rights and documents on a computer.
During the run, DNA is amplified in the reaction vessel, where each sample should be subjected to identical, consistent reaction conditions. The following aspects are relevant for the process:
Excellent accuracy in temperature control and homogeneity of the cycler block are the basis for even temperature conditioning of all samples. The high quality of the heating and cooling elements (peltier elements), as well as the way in which these are connected to the block, are the deciding factors which will determine the risk of temperature discrepancies known as the “edge effect” (figure 7) (Peltiers in PCR)