Productivity

Productivity

The productivity of your PCR experiments can be influenced by many variables like PCR set-up, the runtime of your PCR protocol, and the failure rate of your experiments. Articles here highlight how the planning of experiments, the used processes, consumables, and instruments influences the productivity of PCR workflows.

7 Articles

Multiple targets, one run: multiplex your PCR!

Cross your heart! What would lab life be without PCR? The “good old” PCR has become second nature to us scientists. If you want to get a variety of...

Magnetic beads in NGS library prep

PCR for NGS - Why cyclers and consumables can spoil your run

PCR cyclers and consumables are typical sources of error in next-generation sequencing workflows. The nightmares range from non-specific products,...

Illustration of PCR peltier

Peltiers in PCR matter!

Since the introduction of PCR, thermal cyclers are no longer an unfamiliar piece of equipment to molecular biologists. However, because thermal...

PCR temperature: The meaning of optimal over- and undershoot

PCR temperature: The meaning of optimal over- and undershoot

In a PCR thermal cycler, temperature overshoot and undershoot is a necessary part of temperature cycling because it allows the whole reaction liquid...

Skateboarder on a ramp – how to choose the right thermal cycler from PCR ramp rate specifications

PCR cycler ramp rate: Higher ramp or finish first?

When I was a young and ignorant student, I’ve asked my professor this question: “Why is it that when we say we want a fast cycler, the sales...

Part 2: Speed up your Reaction – Reduction of Preparation Time and Avoiding Runs Entirely

Reducing the time spent on PCR runs is doubtlessly beneficial, as results will be available more quickly and the thermocycler will be free to be used...

Part 1: How do I spend less time on PCR experiments and get results faster?

Much time in the laboratory is spent on conducting PCR experiments. It is worth analyzing which aspects contribute to the total time spent, and where...

7 Articles