PCR is a standard technique among the many methods of molecular biology. It enables amplification of specific regions of DNA in order to utilize them in subsequent analyses or reactions.
While the performance of the reaction, as well as experimental parameters, are important, the ultimate success of the experiment hinges on a number of additional considerations.
Some questions which may arise will be covered by the following articles:
- What is to be considered when designing primers?
- Which components will I need in order to prepare the reaction?
- What is the origin of my sample material?
- Which sample vessel will I use?
- Have I obtained the desired result?
- How will I store sample material and amplified DNA?