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The Electronic Pipette — More Power to You

Every second counts, which is why even small mistakes can lead to serious consequences. You probably handle complex projects, work with small volumes, and take important decisions depending on the results of your work. But is having the perfect tool in your hand enough? The electronic pipette is not only capable of mastering multiple applications precisely, but also helps you achieve highly reproducible results and works time-efficiently. Do not be afraid to use the electronic pipette’s full potential. Read on to find out how.

In the pharmaceutical and Biotech industry, companies are always finding new ways to become more and more efficient to be able to withstand competition and the pressure to perform. Throughput is increasing with the smallest volumes, while efficient performance and high reproducibility has to be maintained in a variety of different applications. To keep the pace up, you need efficient, flexible and highly professional tools. Which is where electronic pipettes can do more for you than you might think.

The following list provides examples of applications that an electronic pipette can help you with to achieve results faster and more accurate. Programming an electronic pipette is pretty straightforward: just select the mode you need and off you go.


Pipetting – Aspirate set volume, and dispense it completely


Applications

  1. For automatic pipetting of all easy and standard pipetting tasks


Automatic pipetting and mixing – Aspirate once, dispense complete volume, and mix it


Applications

  1. For increased number of mixing cycles in general
  2. Re-dissolving of a pellet
  3. Mixing of two liquids e.g. in DNA extraction or PCR-set-up
  4. For standardization of pipetting steps in experiments


Manual pipetting – Aspirate as long as needed, and dispense complete volume


Applications

  1. For very careful uptake of supernatant after centrifugation without disturbing layers
  2. For estimation of an unknown liquid volume
  3.  For careful gel loading (no blow-out)


Dispensing - Aspirate once, dispense the same volume repeatedly (thumb-controlled dispensing)


Applications

  1. Distribution of samples into plates (multi-channel electronic pipette) or larger series of tubes (single-channel electronic pipette), e.g. ELISA or in Molecular Biology (e.g. PCR)
  2. Distribution of master mixes into a plate


Automatic dispensing – Aspirate once, dispense the same volume repeatedly (automatic dispensing with pre-defined time delay)


Applications

  1. Same as for “Dispensing”, but faster and more convenient


Reverse pipetting – Over-aspiration of liquid followed by regular dispensing step


Applications

  1. Transfer of difficult liquids with physical properties other than water
  2. In ELISA applications, for example, the transfer of foam into a microtiter plate can be avoided as well as the formation of bubbles caused by blow-out


Multi-aspiration – Consecutive aspiration of supernatants


Applications

  1. Faster removal of supernatants after centrifugation
  2. Pooling of supernatants to increase material for analysis, e.g. for multiplex sequencing

 

Diluting – Aspiration of a diluent and a sample separated by an air bubble


Applications

  1. For critical reactions and time-dependent measurements that require the mixing of two substances within a certain time


Sequential dispensing – Aspirate once, and dispense several user-defined volumes in sequential order


Applications

Dilution series, e.g. for photometric determination of

  1. DNA quantification
  2. Bradford protein quantification

 

Sequential pipetting – Pipetting of several user-defined volume steps


Applications

  1. For dilution series as described above when

    1. volumes are too large for a sequential dosing method
    2. higher accuracy is needed compared to dispensing mode

  2. For dilution series to quantify DNA at very low detection limits

    1.  e.g. DNA quantification using PicoGreen, or Quant-IT PicoGreen

  3. For simple pipetting protocols
     

Incredible versatility in just one device

Regardless of what type of application you need, it is possible to achieve the perfect result with only one electronic pipette when you select the right mode. Manual pipetting, whether with single or multichannel pipettes, allows you to enter the professional league simply by using electronic pipettes.


Find more information about Eppendorf solutions here

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