Calibration of pipettes is of great importance for accurate and precise pipetting results. Furthermore also the ambient conditions such as air pressure, humidity, temperature and even the altitude affect every pipetting event. Additionally the pipetting technique applied for sample liquids has a huge influence. Challenging liquids such as glycerol, methanol and sulfuric acid need another pipetting technique than water, BSA-solution or medium. Learn about all these factors and how to apply proper techniques for daily pipetting routine as well as the calibration procedure in our texts and video.