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Master of Knowledge
Are you a Master of Knowledge?
Master our pipetting challenge and find out. Once you have mastered a challenge you can print your personalized certificate to proof your abilities. Have fun and develop your skills.
1. What is reverse pipetting?
*
A pipetting technique to handle difficult liquids.
A scheme to pipette samples in reverse order to rule out a pipetting bias.
Taking the sample back out of the vessel you just pipetted in.
2. What happens when you hold a pipette in a substantial angle while aspirating?
*
You aspirate slightly too much liquid.
You aspirate slightly too little liquid.
Nothing, the angle has no influence on the volume.
3. Which effect can liquids with high vapour pressure, e.g. acetone have on air cushion pipetting?
*
None
The vapour can expand the air cushion and leads to dripping.
The vapour can expand the tip, it will fall off.
4. What should you keep in mind when pipetting blood?
*
Keep garlic at hand to fight off vampires.
Use a positive displacement dispenser to dispense accurate volumes.
When finished, clean the pipette tips properly before reusing.
5. Is pre-wetting of pipette tips recommended?
*
No
Yes, but only for volumes < 10µL
Yes, in general
6. Which material are most pipette tips made of?
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Polypropylene
Polycarbonate
Polystyrene
7. Why should pipette tip and pipette from the same manufacturer be used for calibration?
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Because the tips have the required length of 1/5 of the pipette
Because they are made from the same material e.g., polypropylene
Because they are harmonized with each other and build a system
8. What is the purpose of the filter in filter tips?
*
To prevent parts from the pipette to fall into your samples.
Protecting the pipette and the sample from aerosols and biomolecules.
Create a laminar air flow in the pipette tip.
9. What is the right immersion depth to aspirate liquids?
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Dive! Go all the way to the bottom.
Somewhere in the middle should be fine.
As little as possible.
10. What happens to the aspirated volume of the remaining channels if you do not use all channels of a multichannel pipette?
*
The aspirates volume in the remaining channels will increase, proportionally the number of unused channels.
The aspirated volumes depend on the distance the piston moves. Thus, the accuracy and precision are not affected.
The aspirates volume in the remaining channels will decrease, proportionally the number of unused channels.
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