In order to prevent contaminations in cell cultures, it is imperative that equipment be thoroughly cleaned, or exchanged, after work with each cell line. Sterile technique is still the overarching principle wherever cell culture is concerned. Many obvious and perhaps not quite so obvious potential sources of contamination exist in the lab. As mentioned earlier, contamination is not limited to contamination with prokaryotic cells or fungi, but it also includes cross-contamination with cells from other cell lines.
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